Publications

Names of ILS employees are shown in boldface font:

Assessment of Naphthalene-Induced Genotoxicity & Gluathione Detoxication in Human TK6 Lymphoblasts


Recio L, Shepard KG, Hernandez, LG, Kedderis GL. 2012. Dose-Response Assessment of Naphthalene-Induced Genotoxicity and Glutahione Detoxication in Human TK6 Lymphoblasts. Toxicol. Sci. 126(2):405-412.



Therapeutic Ultrasound as a Potential Male Contraceptive


Tsuruta JK, Dayton PA, Gallippi CM, O'Rand MG, Streicker MA, Gessner, RC, Gregory TS, Silva E JR, Hamil, KG, Moser GJ, Sokai, DC. 2012. Therapeutic Ultrasound as a Potential Male Contraceptive: Power, Frequency and Temperature Required to Deplete Rat Testes of Meiotic Cells and Epididymides of Sperm Determined Using Commercially Available System. Reproductive Biology and Endocrinology 10:7.



15-Day Intact Male Rat Assay: Evaluation of an Antithyroid Chemical and a Negative Control Chemical


Becker RA, Bergfelt, DR, Borghoff S, Davis JP, Hamby BT, O'Connor JC, Kaplan AM, Sloan CS, Tyl, RW, Wade M, Marty MS. 2012. Interlaboratory Study Comparison of the 15-Day Intact Adult Male Rat Screening Assay: Evaluation of an Antithyroid Chemical and a Negative Control Chemical. Birth Defects Research (Part B). (95):63-78.



Intestinal Lymphangiectasis and Lipidosis in Rats Following Subchronic Exposure to Indole-3-Carbinol


Boyle MC, Crabbs TA, Wyde ME, Painter TJ, Hill GD, Malarkey DE, Lieuallen WG, Nyska A. 2012. Intestinal Lymphangiectasis and Lipidosis in Rats Folloing Subchronic Exposure to Indole-3-Carbinol via Oral Gavage. Toxicol. Pathol. DOI: 10.1177/0192623311436178:1-16.



Improving Animal Welfare and Reducing Animal Use for Veterinary Vaccine Potency Testing


Stokes WS, Brown K, Kulpa-Eddy J, Srinivas G, Halder M, Draayer H, Galvin J, Claasen I, Gifford G, Woodland R, Doelling V, Jones B. 2011. Improving Animal Welfare and Reducing Animal Use for Veterinary Vaccine Potency Testing: State of the Science and Future Directions. Procedia in Vaccinology(5):84-105.



Alt. Methods to Reduce, Refine & Replace Animal Use for Veterinary Vaccine Post-Licensing Testing


Kulpa-Eddy J, Srinivas G, Halder M, Brown K, Draayer H, Glavin J, Claassen I, Gifford G, Woodland R, Doelling V, Jones, B, Stokes WS. 2011. Alternative Methods and Strategices to Reduce, Refine and Replace Animal Use for Veterinary Vaccine Post-Licensing Safety Testing: State of the The Science and Future Directions.Procedia in Vaccinology(5):106-119.



Alternative Methods to Reduce, Refine & Replace Animal Use for Human Vaccine Post-Licensing Testing


Isbrucker R, Levis R, Casey W, McFarland R, Schmitt M, Arciniega J, Descamps J, Finn T, Hendriksen C, Horiuchi Y, Keller J, Kojima H, Sesardic D, Stickings P, Johnson NW, Allen D. 2011. Alternative Methods and Strategies to Reduce, Refine and Replace Animal Use for Human Vaccine Post-Licensing Safety Testing: State of the Science and Future Directions. Procedia in Vaccinology(5):47-59.



Non-Animal Replacement Methods for Veterinary Vaccine Potency Testing


Kulp-Eddy J, Srinivas G, Halder M, Hill R, Brown K, Roth J, Draayer H, Galvin G, Woodland R, Doelling V, Jones B, Stokes WS. 2011. Non-Animal Replacement Methods for Veterinary Vaccine Potency Testing: State of the Science and Future Directions. Procedia in Vaccinology(5):60-83.



Improving Animal Welfare and Reducing Animal Use for Human Vaccine Potency Testing


Casey W, Schmitt M, McFarland R, Isbrucker R, Levis R, Arciniega J, Descamps J, Finn T, Hendriksen C, Horluchi Y, Keller, J, Kojima H, Sesardic D, Stickings P, Johnson NW, Lipscomb E, Allen D. 2011. Improving Animal Welfare Reducing Animal Use for Human Vaccine Potency Testing: State of the Science and Future Directions. Procedia in Vaccinology(5):33-46.



Non-Animal Replacement Methods for Human Potency Testing: State of the Science and Future Directions


McFarland R, Verthelyi D, Casey W, Arciniega J, Isbrucker R, Schmitt M, Finn T, Descamps J, Horiuchi Y, Sesardic D, Stickings P, Johnson NW, Lipscomb E, Allen D. 2011. Non-Animal Replacement Methods for Human Vaccine Potency Testing: State of the Science and Future Directions. Procedia in Vaccinology(5): 16-32.



Evaluation of the Genotoxicity of the Food Additive, Gum Ghatti


Hobbs CA, Swartz C, Maronpot R, Davis J, Recio L, and Hayashi S. 2011. Evaluation of the Genotoxicity of the Food Additive, Gum Ghatti. Food and Chemical Toxicology DOI:10.1016/j.fct.2011.11.021.



Improvement of In Vivo Genotoxicity: Acute Tests and Integration into Standard Toxicity Testing


Rothfuss A, Honma M, Czich A, Aardema MJ, Burlinson B, Galloway S, Hamada S, Kirkland D, Heflich RH, Howe J, Nakajima M, O'Donovan M, Plappert-Helbig U, Priestley C, Recio L, Schuler M, Uno Y, and Martus HJ. 2011. Improvement of In Vivo Genotoxicity: Combination of Acute Tests and Integration into Standard Toxicity Testing. Mutat. Res. 723: 108-120.



Comet Assay Dose-Response for Ethyl Methanesulfonate Using Freshly Prepared Vs. Cryopreserved Tissue


Recio L, Kissling GE, Hobbs CA, and Witt KL, 2011. Comparison of Comet Assay Dose-Response for Ethyl Methansulfonate Using Freshly Prepared Versus Cryopreserved Tissues. Environmental and Molecular Mutagenesis (in press).



Using Fewer Animals to Identify Chemical Eye Hazards: Criteria to Maintain Hazard Classification


Haseman JK,Allen DG,Lipscomb EA,Truax JF,Stokes WS. 2011. Using Fewer Animals to Identify Chemical Eye Hazards: Revised Criteria Necessary to Maintain Equivalent Hazard Classification. Regulatory Toxicology and Pharmacology 61:98-104.



Disseminiated Histoplasmosis with Concurrent Oral Candidiasis in an Eclectus Parrot


Quist EM,Belcher C,Levine G,Johnson M,Heatley JJ,Kiupel M, Giri D. 2011. Disseminated Histoplasmosis with Concurrent Oral Candidiasis in an Electus Parrot (Eclectus Roratus). Avian Pathol. 40(3):339.



Cerebral and Renal Phaeohyphomycosis in a Dog Infected with Bipolaris Species


Giri DK,Sims WP,Sura R,Cooper JJ,Gavrilov BK,Mansell J. 2011. Cerebral and Renal Phaeohhyphomycosis in a Dog Infected with Bipolaris Species. Vet Pathol. 48(3):754-7.



Neoplastic and Nonneoplastic Liver Lesions Induced by Dimethylnitrosamine in Japanese Medaka Fish


Hobbie KR,DeAngelo AB,George MH,Law JM. 2011. Neoplastic and Nonneoplastic Liver Lesion Induced by Dimethylnitrosamine in Japanes Medaka Fish. Vet. Pathol. Online. DOI: 10.1177/0300985811409443.



Liver Toxicity and Carcinogenicity in f344/n Rats and b6cf1 Mice Exposed to Kava Kava


Behl M,Nyska A,Chhabra RS,Travlos G,Fomby LM,Sparrow BR,Hejtmancik MR,Chan PC. 2011. Liver Toxicity and Carcinogenicity in f344/n Rats and b6c3f1 Mice Exposed to Kava Kava. Food and Chemical Toxicology. In press for publication in November 2011.



Safety Assessment of Allergic Contact Dermatitis Hazards:  An Analysis Supporting Reduced Animal Use


Haseman JK,Strickland J,Allen D,Salicru E,Paris M,Tice RR,Stokes WS. 2011. Safety Assessment of Allergic Contact Dermatitis Hazards: An Analysis Supporting Reduced Animal Use for the Murine Local Lymph Node Assay. Regulatory Toxicology and Pharmacology 59: 191-96.



Assessment of a Regulated AAV2 Gene Transfer Vector After Murine Submandibular Glands Delivery


Zheng C,Voutetakis A,Goldstein B,Afione S,Rivera V,Clackson T,Wenk M,Boyle M,Nyska A,Chiorini J,Vallant M,Irwin R,Baum B. 2011. Assessment of the Safety and Biodistribution of a Regulated AAV2 Gene Transfer Vector After Delivery to Murine Submandibular Glands. Toxicological Sciences.



Effects of Atrazine on Steroidgenesis in Rat Granulosa and H295R Adrenal Cortical Carcinoma Cells


Tinfo NS,Hotchkiss MG,Buckalew AR,Zorrilla LM,Cooper RL,Laws SC. 2011. Understanding the Effects of Atrazine on Steriodogenesis in Rat Granulosa and H295 Adrenal Cortical Carcinoma Cells.Reprod. Toxicol. 31(2):184-93



Histopathological Evaluation of the Nervous System in National Toxicology Program of Rodent Studies


Rao, D, Little, PB, Malarkey, D.E, Herbert, RA, Sills, RC. 2011. Histopathological Evaluation of the Nervous System in National Toxicology Program of Rodent Studies: A Modified Approach. Toxicologic Pathology 39: 463-470.



Genetic Toxicology in the 21st Century: Reflections and Future Directions


Mahadevan B, Snyder R, Waters M, Benz RD, Kemper RA, Tice RR and Richard AM. 2011. Genetic Toxicology in the 21st Century: Reflections and Future Directions. Environ. Mol. Mutagen. 52.



Effects of Hormonally Active Agents on Steroid Hormone Receptor Expression and Cell Proliferation


Hill GD, Moore AB, Kissling GE, Flagler ND, Ney E, Cline JM, Dixon D. 2011. Effects of Hormonally Active Agents on Steroid Hormone Receptor Expression and Cell Proliferation in the Myometrium of Ovariectomized Macaques. Toxicol. Pathol. 39(3): 508-515.



Toxicity Study of Green Tea Extract in Rats and Mice


Chan PC, Ramot Y, Malarkey DE, Blackshear P, Kissling GE, Travlos G, Nyska A. 2010. Fourteen week Toxicity Study of Green Tea Extract in Rats and Mice. Toxicol. Pathol. 38(7): 1070-1084.



Characterizing and Predicting Carcinogenicity and Mode of Action


Waters M, Jackson MA, and Lea IA. 2010. Characterizing and predicting carcinogenicity and mode of action using conventional and toxicogenomics methods. Mutat Res 705: 184-200.



Dose-Response of Four Genotoxic Chemicals in a Combined Mouse and Rat Micronucleus and Comet Assay


Recio L, Hobbs CA, Caspary W, Witt KL. 2010. Dose-Response Assessment of Four Genotoxic Chemicals in a Combined Mouse and Rat Micronucleus and Comet Assay Protocol. Toxicol Sciences 35(2): 149-162.



No Biomarkers of Genetic Damage or Pathological Changes in Male Rats Administered Ritalin


Witt KL, Malarkey DE, Hobbs CA, Davis JP, Kissling GE, Caspary W, Travlos G, Recio L. 2010. No increases in biomarkers of genetic damage or pathological changes in heart and brain tissues in male rats administered methylphenidate hydrochloride (Ritalin) for 28 days. Environ Mol Mutagen 51(1): 80-88.



Useful Immunohistochemical Markers of Tumor Differentiation


Painter JT, Clayton MP and Herbert RA. 2010. Useful Immunohistochemical Markers of Tumor Differentiation. Toxicol. Pathol. 38(1): 131-141.



Genetic Pathways to Colorectal Cancer


Lea IA, Jackson MA and Dunnick JK. 2009. Genetic Pathways to Colorectal Cancer. Mutation Research 670(1-2): 96-8.



Methyl Isobutyl Ketone (MIBK) Induction of a2u-globulin Nephropathy in Male, but not female Rats


Borghoff SJ, Hard GC, Berdasco NA, Gingell R, Green SM, and Gulledge W. 2009. Methyl isobutyl ketone (MIBK) induction of a2u-globulin nephropathy in male, but not female rats. Toxicology 258(2-3): 131-8.



Pharmacokinetic Model of Methyl Tertiary Butyl Ether and Tertiary Butyl Alcohol Binding


Leavens T and Borghoff SJ. 2009. Physiologically Based Pharmacokinetic Model of Methyl Tertiary Butyl Ether and Tertiary Butyl Alcohol with Binding to a2u-Globulin in the Kidney. Toxicol. Sciences 109(2): 321-335.



Furan-Induced Dose-response for Liver Cytotoxicity, Cell Proliferation, and Tumorigenicity


Moser GJ, Foley J, Burnett M, Goldsworthy TL, Maronpot R. 2009. Furan-induced dose response relationships for liver cytotoxicity, cell proliferation, and tumorigenicity (furan-induced liver tumorigenicity). Exp. Toxicol. Pathol. 61(2): 101-11.



Improving Prediction of Chemical Carcinogenicity Considering Multiple Mechanisms


Guyton KZ, Kyle AD, Aubrecht J, Cogliano VJ, Eastmond DA, Jackson MA, Keshava N, Sandy MS, Sonawane B, Zhang L, Waters MD and Smith MT. 2009. Improving Prediction of Chemical Carcinogenicity by Considering Multiple Mechanisms and Applying Toxicogenomic Approaches. Mutat Res 681: 230-240.



Kinetics of Selected Di-n-butyl Phthalate Metabolites and Fetal Testosterone in Pregnant Rats


Clewell RA, Kremer JJ, Williams CC, Campbell, Jr. JL, Sochaski M, Andersen ME, and Borghoff SJ. 2009. Kinetics of selected di-n-butyl phthalate metabolites and fetal testosterone following repeated and single administration in pregnant rats. Toxicology. 255(1-2): 80-90.



Investigation of the Low-Dose Response in In Vivo Induction of Micronuclei and Adducts by Acrylamide


Zeiger E, Recio L, Fennell TR, Haseman JK, Snyder RW, and Friedman M. 2009. Investigation of the Low-Dose Response in the In Vivo Induction of Micronuclei and Adducts by Acrylamide Toxicological Sciences. Experimental and Toxicologic Pathology. 107(1): 247-157.



Databases Applicable to Quantitative Hazard/Risk Assessment


Waters M and Jackson MA. 2008. Databases Applicable to Quantitative Hazard/Risk Assessment -- Towards a Predictive Systems Toxicology. Toxicol. Appl. Pharmacol. in press.



Furan-Induced Dose Response Relationships for Liver


Moser GJ, Foley J, Burnett M, Goldsworthy TL, Maronpot R. 2008. Furan-Induced Dose Response Relationships for Liver Cytotoxicity, Cell Proliferation, and Tumorigenicity. Exp. Toxicol. Pathol.



Characterization of Uterine Granular Cell Tumors in Mice


Veit AC, Painter JT, Miller RA, Hardisty JF, and Dixon D. 2008. Characterization of Uterine Granular Cell Tumors in B6C3F1 Mice: A Histomorphologic, Immunohistochemical, and Ultrastructural Study. Vet Pathol, 45: 654-662.



Toward a Molecular Equivalent Dose: Use of the Medaka Model in Comparative Risk Assessment.


Hobbie KR, DeAngelo AB, King LC, Winn RN, Law M. 2009. Toward a molecular equivalent dose: Use of the Medaka model in comparative risk assessment. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 149(2): 141-151.



Tissue Exposures to Free and Glucuronidated Monobutylphthalate in Pregnant and Fetal Rats


Clewell RA, Kremer JJ, Williams CC, Campbell JL, Andersen ME, Borghoff SJ. 2008. Tissue Exposures to Free and Glucuronidated Monobutylphthalate in Pregnant and Fetal Rat Following Exposure to di-n-Butylphthalate: Evaluation with a PBPK Model. Toxicol. Sci. 103(2): 241-59.



Genotoxicity of 10 Cigarette Smoke Condensates in Four Test Systems


Demarini DM, Gudi R, Szkudlinska A, Rao M, Recio L, Kehl M, Kirby PE, Polzin G, Richter PA. 2008. Genotoxicity of 10 Cigarette Smoke Condensates in Four Test Systems: Comparisons Between Assays and Condensates. Mutat. Res. 650(1): 15-29.



The Environmental Polymorphisms Registry: A DNA Resource to Study Genetic Susceptibility Loci


Chulada PC, Vahdat HL, Sharp RR, DeLozier TC, Watkins PB, Pusek SN, Blackshear PE. 2008. The Environmental Polymorphisms Registry: A DNA Resource to Study Genetic Susceptibility Loci. Hum. Genet. 123(2): 207-14.



Comparison of Flow Cytometry and Microscopy-Based Methods for Measuring Micronucleated Reticulocyte


Witt KL, Livanos E, Kissling GE, Torous DK, Caspary W, Tice RR, Recio L. 2008. Comparison of Flow Cytometry and Microscopy-Based Methods for Measuring Micronucleated Reticulocyte Frequencies in Rodents Treated with Nongenotoxic and Genotoxic Chemicals. Mutat. Res. 649(1-2): 101-13.



Toxicity and Carcinogenicity Studies of 4-Methylimidazole in F344/N Rats and B6C3F1 Mice


Chan PC, Hill GD, Kissling GE, and Nyska A. 2008. Toxicity and Carcinogenicity Studies of 4-Methylimidazole in F344/N Rats and B6C3F1 Mice. Archives of Toxicology 82(1):45-53.



Subchronic Administration of Indole-3-Carbinol in B6C3F1 Mice and Fischer 344 Rats


Wyde ME, Bordelon NR, Mann J, Hill G, Painter JT, Yoshizawa K, Hebert CD, Bucher JR, and Nyska A. 2008. Subchronic Administration of Indole-3-Carbinol in B6C3F1 Mice and Fischer 344 Rats Is Associated with Increased Hepatic CYP 1A1 and 1A2 Activities, but without Liver Toxicity. Toxicol Pathol 36(1):168.



CEBS—Chemical Effects in Biological Systems: A Public Data Repository


Waters M, Stasiewicz S, Merrick BA, Tomer K, Bushel P, Paules R, Stegman N, Nehls G, Yost KJ, Johnson CH, Gustafson SF, Xirasagar S, Xiao N, Huang CC, Boyer P, Chan DD, Pan Q, Gong H, Taylor J, Choi D, Rashid A, Ahmed A, Howle R, Selkirk J, Tennant R and Fostel J. 2008. CEBS--Chemical Effects in Biological Systems: A public Data Repository Integrating Study Design and Toxicity Data with Microarray and Proteomics Data Nucleic Acids Res, 36, D892-900.



Bayesian Estimation of Pharmacokinetic and Pharmacodynamic Parameters


Liao KH, Tan Y, Conolly RB, Borghoff SJ, Gargas ML, Andersen ME, and Clewell HJ. 2007. Bayesian Estimation of Pharmacokinetic and Pharmacodynamic Parameters in a Mode-of-Action-Based Cancer Risk Assessment for Chloroform. Risk Analysis 27, 1535-1551.



14C-Labeled Pulegone and Metabolites Binding to alpha2u-Globulin in Kidneys of Male F-344 Rats


Ferguson LJ, Lebetkin EH, Lih FB, Tomer KB, Parkinson HD, Borghoff SJ, and Burka LT. 2007. 14C-Labeled Pulegone and Metabolites Binding to alpha2u-Globulin in Kidneys of Male F-344 Rats. J Toxicol. Environ. Health A. 70(17): 1416-1423.



Polyamine-Mediated Regulation of Protein Acetylation in Murine Skin and Tumors


Wei G, Hobbs CA, Defeo K, Hayes CS, Gilmour SK. 2007. Polyamine-Mediated Regulation of Protein Acetylation in Murine Skin and Tumors. Mol. Carcinog. 46(8): 611-7.



Consensus Recommendations to Promote and Advance Predictive Systems Toxicology and Toxicogenomics


Waters M and Yauk C. 2007. Consensus Recommendations to Promote and Advance Predictive Systems Toxicology and Toxicogenomics. Environ. Mol. Mutagen, 48(5): 400-3.



Alpha 2u-globulin Nephropathy and Renal Tumors in National Toxicology Program Studies


Doi AM, Hill G, Seely J, Hailey JR, Kissling G, and Bucher JR. 2007. Alpha 2u-globulinNephropathy and Renal Tumors in National Toxicology Program Studies. Toxicol. Pathol. 35(4):533-540.



Fetal Mouse Phthalate Exposure


Gaido KW, Hensley JB, Liu D, Wallace DG, Borghoff SJ, Johnson KJ, Hall SJ, Boekelheide K. 2007. Fetal Mouse Phthalate Exposure Shows that Gonocyte Multinucleation is Not Associated with Decreased Testicular Testosterone. Toxicol Sciences 97(2):491-503.



Methyl tertiary-butyl ether Mode of Action for Cancer Endpoints in Rodents


Cruzana G, Borghoff SJ, Peysterc A, Hardd GC, McClaine M, McGregor DB and Thomas MG. 2007. Methyl tertiary-butyl ether Mode of Action for Cancer Endpoints in Rodents. Regul. Toxicol. Pharmicol. 47(2): 156-65.



Hepatic Transcript Levels for Genes Coding for Enzymes Associated with Xenobiotic Metabolism


Mori K, Blackshear PE, Lobenhofer EK, Parker JS, Orzech DP, Roycroft JH, Walker KL, Johnson KA, Marsh TA, Irwin RD and Boorman GA. 2007. Hepatic Transcript Levels for Genes Coding for Enzymes Associated with Xenobiotic Metabolism are Altered with Age. Toxicol. Pathol. 35(2): 242-51.



Application of Visualization Tools to the Analysis of Histopathological Data


Lobenhofer EK, Boorman GA, Phillips KL, Heinloth AN, Malarkey DE, Blackshear PE, Houle C and Hurban P. 2006. Application of Visualization Tools to the Analysis of Histopathological Data Enhances Biological Insight and Interpretation. Toxicol. Pathol. 34(7): 921-8.



Heparin and Fibroblast Growth Factors Affect Surfactant Protein Gene Expression in Type II Cells


Leiner KA, Newman D, Li CM, Walsh E, Khosla J, Sannes PL. 2006. Heparin and Fibroblast Growth Factors Affect Surfactant Protein Gene Expression in Type II Cells. Am J Respir. Cell. Mol. Biol. 35(5): 611-8.



Tip60 Protein Isoforms and Altered Function in Skin and Tumors


Hobbs CA, Wei G, DeFeo K, Paul B, Hayes CS and Gilmour SK. 2006. Tip60 Protein Isoforms and Altered Function in Skin and Tumors that Overexpress Ornithine Decarboxylase. 66: 8116-8122.



Genetic Alterations in Cancer Knowledge System: Analysis of Gene Mutations in Mouse and Human Liver


Jackson MA, Lea I, Rashid A, Peddada SD, Dunnick JK. 2006. Genetic Alterations in Cancer Knowledge System: Analysis of Gene Mutations in Mouse and Human Liver and Lung Tumors. Toxicol. Sci. 90 (2): 400-418.



Toxicogenomics and the Evolution of Systems Toxicology


Waters M and Merrick BA. 2004. Toxicogenomics and the Evolution of Systems Toxicology. General and Applied Toxicology, 3rd Edition, B. Ballantyne, T. Marrs, and T. Syversen, Eds., John Wiley & Sons, Ltd, Chichester, United Kingdom.



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