Publications

Names of ILS employees are shown in boldface font:

Open-source Workflows for In Vitro to In Vivo Extrapolation


X Chang, SM Bell, JR Pirone, D Allen, W Casey, N Kleinstreuer. 2017. Open-source Workflows for In Vitro to In Vivo Extrapolation. (Abstract # 3185)



Development of a Curated Database of In Vivo Developmental Toxicity Data


P Ceger, N Choksi, J Hamm, J Truax, A Daniel, D Allen, V Sutherland, B McIntyre, P Foster, E Maull, W Casey. 2017. Development of a Curated Database of In Vivo Developmental Toxicity Data. (Abstract #3159)



An Integrated Chemical Environment to Support 21st Century Toxicology


SM Bell, J Phillips, A Sedykh, C Sprankle, S Morefield, D Allen, W Casey, N Kleinstreuer. 2017. An Integrated Chemical Environment to Support 21st Century Toxicology. (Abstract # 2935)



Prediction of Skin Sensitization Potency Using Machine Learning Approaches


J Strickland, Q Zang, M Paris, DM Lehmann, S Bell, D Allen, J Matheson, A Jacobs, W Casey, N Kleinstreuer. 2017. Prediction of Skin Sensitization Potency Using Machine Learning Approaches. (Abstract #2915)



Identification, Curation, and Prioritization of Food-Use Chemicals in ToxCast


M Krishan, TD Trautman, DF Kapraun, LA Fix, and AL Karmaus. 2017. Identification, Curation, and Prioritization of Food-Use Chemicals in ToxCast. (Abstract #2559)



In Vitro Micronucleus and CometChip with Metabolically Competent HepaRG Cells


Swartz, CD, Winters, J, Owens, K, Yauk, CL, Buick, J, Ngo, L, Engelward, B, and Recio, L. 2017. In Vitro Micronucleus® and CometChip with Metabolically Competent HepaRG™ Cells. (Abstract # 2267)



Acrylamide Perturbs Calcium and Actin Cytoskeleton Signaling in Rat and Mouse Cancer Target Tissues


Chepelev NL, Gagné R, Maynor T, Hobbs CA, Kuo B, Yauk CL, Recio L. 2017. Acrylamide Perturbs Calcium and Actin Cytoskeleton Signaling in Rat and Mouse Cancer Target Tissues. (Abstract # 1830)



Customizing the Connectivity Map Approach for Functional Evaluation in Toxicogenomics Studies


Karmaus AL, Kothiya P, Watford S, Thomas RS, Martin MT. 2017. Customizing the Connectivity Map Approach for Functional Evaluation in Toxicogenomics Studies. (Abstract #1374)



Toxicogenomics Reveals Dynamic Baseline Changes and Overlapping Effects of Epigenotoxicant…


Hobbs CA, Sinha N, Chepelev N, Gagné R, Christy N, Yauk CL, Recio L. 2017. Toxicogenomics Reveals Dynamic Baseline Changes and Overlapping Effects of Epigenotoxicant Exposure on Stem Cell Differentiation and Tissue Development Networks. (Abstract #1344)



Ninety-day toxicity and single-dose toxicokinetics study of AGIQ in Sprague-Dawley rats


Nyska A, Hayashi S, Koyanagi M, Davis J, Jokinen M, Ramot Y, Maronpot R. 2016. Ninety-day toxicity and single-dose toxicokinetics study of alphaglycosyl isoquercitrin in Sprague-Dawley rats. Food and Chemical Toxicology. 2016 Sep 27. pii: S0278-6915(16)30346-5.



Genotoxicity assessment of the flavouring agent, perillaldehyde


Hobbs C, Taylor S, Beevers C, Lloyd M, Bowen R, Lillford L, Maronpot R, Hayashi S. 2016. Genotoxicity assessment of the flavouring agent, perillaldehyde. Food and Chemical Toxicology. 2016 Sep 1;97:232-242. doi: 10.1016/j.fct.2016.08.029.



Chemical Reactivity and Respiratory Toxicity of the alpha-Diketone Flavoring Agents


Morgan D, Jokinen M, Johnson C, Price H, Gwinn W, Bousquet R, Flake G. 2016. Chemical Reactivity and Respiratory Toxicity of the alpha-Diketone Flavoring Agents: 2,3-Butanedione, 2,3-Pentanedione, and 2,3-Hexanedione. Toxicologic Pathology. 2016 Jul;44(5):763-83. doi: 10.1177/0192623316638962.



Differential genotoxicity of acrylamide in the MN and Pig-a assays in F344 rats and B6C3F1 mice


Hobbs CA, Davis J, Shepard K, Chepelev N, Friedman M, Marroni D, Recio L. 2016. Differential genotoxicity of acrylamide in the micronucleus and Pig-a gene mutation assays in F344 rats and B6C3F1 mice. Mutagenesis. 2016 Jun 23. pii: gew028.



Advancing toxicology research using in vivo high throughput toxicology with small fish models


Planchart A, Mattingly CJ, Allen D, Ceger P., Casey W, Hinton D, Kanungo J, Kullman SW, Tal T, Bondesson M, Burgess SM, Sullivan C, Kim C, Behl M, Padilla S, Reif DM, Tanquay RL, Hamm J. 2016. Advancing toxicology research using in vivo high throughput toxicology with small fish models. Altex. 2016 Jun 21. doi: 10.14573/altex.1601281.



Integrated decision strategies for skin sensitization hazard


Strickland J, Zang Q, Kleinstreuer N, Paris M, Lehmann DM, Choksi N, Matheson J, Jacobs A, Lowit A, Allen D, Casey W. 2016. Integrated decision strategies for skin sensitization hazard. Journal of Applied Toxicology. 2016 Feb 6. doi: 10.1002/jat.3281.



Accelerating adverse outcome pathway development using publicly available data sources


Oki NO, Nelms MD, Bell SM, Mortensen HM, Edwards SW. 2016. Accelerating adverse outcome pathway development using publicly available data sources. Curr Environ Health Rep. 2016 Mar;3(1):53-63.



Adverse Outcome Pathways: From Research to Regulation. Scientific workshop report


Kleinstreuer NC, Sullivan K, Allen D, Edwards S, Mendrick DL, Embry M, Matheson J, Rowlands JC, Munn S, Maull E, Casey W. 2016. Adverse Outcome Pathways: From Research to Regulation. Scientific workshop report. Regulatory Toxicology & Pharmacology. 2016 Jan 14;76:39-50 doi: 10.1016/j.yrtph.2016.01.007



Enhanced stability of TTP mRNA protects mice against immune-mediated inflammatory pathologies


Patial S, Curtis AD, Lai WS, Stumpo DJ, Hill GD, Flake GP, Mannie MD, Blackshear PJ. Enhanced stability of tristetraprolin mRNA protects mice against immune-mediated inflammatory pathologies. Proc Natl Acad Sci U S A. 2016 Feb 1. pii: 201519906.



Performance of the BG1Luc ER TA method in a qHTS format


Ceger P, Allen D, Huang R, Xia M, Casey W. Performance of the BG1Luc ER TA method in a qHTS format. ALTEX. 2015 Jun 28. doi: 10.14573/altex.1505121



Gene Expression Patterns Associated With Histopathology in Toxic Liver Fibrosis


Ippolito DL, AbdulHameed MD, Tawa GJ, Baer CE, Permenter MG, McDyre BC, Dennis WE, Boyle MH, Hobbs CA, Streicker MA , Snowden BS, Lewis JA, Wallqvist A, Stallings JD.Gene expression patterns associated with histopathology in toxic liver fibrosis. Toxicol Sci. 2015 Sep 22. pii: kfv214.



Bayesian Integrated Testing Strategy (ITS) for Skin Sensitization Potency Assessment


Jaworska JS,· Natsch A, Ryan C, · Strickland J, ·Ashikaga T, · Miyazawa M. 2015. Bayesian integrated testing strategy (ITS) for skin sensitization potency assessment: a decision support system for quantitative weight of evidence and adaptive testing strategy. Arch Toxicol. 2015 Dec; doi: 10.1007/s00204-015-1634-2



Utilizing Whole Slide Images for Pathology Peer Review and Working Groups


Malarkey DE, Willson GA, Willson CJ, Adams ET, Olson GR, Witt WM, Elmore SA, Hardisty JF, Boyle MC, Crabbs TA, Miller RA. (2015) Utilizing Whole Slide Images for Pathology Peer Review and Working Groups. Toxicol Pathol. 2015 Oct 27. pii: 0192623315605933



IHC Expression of Cyclin D1, Cytokeratin 20, & Uroplakin III Induced by o-Nitroanisole


Cynthia J. Willson, Gordon P. Flake, Robert C. Sills, Grace E. Kissling, and Mark F. Cesta. (2015) Immunohistochemical Expression of Cyclin D1, Cytokeratin 20, and Uroplakin III in Proliferative Urinary Bladder Lesions Induced by o-Nitroanisole in Fischer 344/N Rats. Veterinary Pathology, first published on August 28, 2015 as doi:10.1177/0300985815603432



Integration of metabolic activation w TGx signature to classify genotoxic vs nongenotoxic chemicals


J.K. Buick, I. Moffat, A. Williams, C.D. Swartz, L. Recio, D. R. Hyduke, H.H. Li, A.J. Fornace Jr, J. Aubrecht, C.L. Yauk. (2015) Integration of metabolic activation with a predictive toxicogenomics signature to classify genotoxic versus nongenotoxic chemicals in human TK6 cells. Environ Mol Mutagen 56:520–534. doi.org/10.1002/em.21940. PMID: 25733247



A predictive TGx signature to classify genotoxic vs non-genotoxic chemicals in human TK6 cells


Andrew Williams, Julie K. Buick, Ivy Moffat, Carol D. Swartz, Leslie Recio, Daniel R. Hyduke, Heng-Hong Li, Albert J. Fornace Jr., Jiri Aubrecht, Carole L. Yauk. (2015) A predictive toxicogenomics signature to classify genotoxic versus non-genotoxic chemicals in human TK6 cells. Data in Brief. doi:10.1016/j.dib.2015.08.013



Impact of Genomics Platform & Statistics on Transcriptional BMD & Selection of Chemical PoD


Webster AF, Chepelev N, Gagné R, Kuo B, Recio L, Williams A, Yauk CL. (2015) Impact of Genomics Platform and Statistical Filtering on Transcriptional Benchmark Doses (BMD) and Multiple Approaches for Selection of Chemical Point of Departure (PoD). PLoS One. Aug 27;10(8):e0136764. doi: 10.1371/journal.pone.0136764. PMID: 26313361



TGx & traditional approaches to inform MoA and PoD in health risk assessment of benzo[a]pyrene


Moffat I, Chepelev NL, Labib S, Bourdon-Lacombe J, Kuo B, Buick JK, Lemieux F, Williams A, Halappanavar S, Malik AI, Luijten M, Aubrecht J, Hyduke DR, Fornace AJ Jr, Swartz CD, Recio L, Yauk CL. (2015) Comparison of toxicogenomics and traditional approaches to inform mode of action and points of departure in human health risk assessment of benzo[a]pyrene in drinking water. Crit Rev Toxicol. 45(1):1-43. doi: 10.3109/10408444.2014.973934. Review. PMID: 25605026



BrdU treatment to measure hepatocellular proliferation does not mask furan-induced mRNA in mouse


Webster AF, Williams A, Recio L, Yauk CL. (2014) Bromodeoxyuridine (BrdU) treatment to measure hepatocellular proliferation does not mask furan-induced gene expression changes in mouse liver. Toxicology. 323:26-31. doi: 10.1016/j.tox.2014.06.002. PMID: 24910943



Mining the archives: a cross-platform analysis of gene expression profiles in archival FFPE tissue


Webster AF, Zumbo P, Fostel J, Gandara J, Hester SD, Recio L, Williams A, Wood CE, Yauk CL, Mason CE. (2015) Mining the archives: a cross-platform analysis of gene expression profiles in archival formalin-fixed paraffin-embedded (FFPE) tissue. Toxicol Sci. [Epub ahead of print] PMID: 26361796



Safety assessment and single-dose toxicokinetics of the flavouring agent, myricitrin, in rats


Robert R. Maronpot, Mihoko Koyanagi, Jeffrey Davis, Leslie Recio, Dean Marbury, Molly Boyle & Shim-Mo Hayashi. (2015) Safety assessment and single-dose toxicokinetics of the flavouring agent, myricitrin, in Sprague Dawley rats. Food Additives & Contaminants: Part A (2015).



Genotoxicity evaluation of the flavonoid, myricitrin, and its aglycone, myricetin


Hobbs CA, Swartz C, Maronpot R, Davis J, Recio L , Koyanagi M, Hayashi SM. (2015) Genotoxicity evaluation of the flavonoid, myricitrin, and its aglycone, myricetin. Food and Chemical Toxicology 83 (2015) 283-292.



Histopathological and Immunohistochemical Characterization of Cell Leisons in Stomach of Rats


Janardhan KS , Rebolloso Y, Hurlburt G, Olson D, Lyght O, Clayton NP, Gruebbel M, Picut C, Shackelford C, Herbert RA. (2015) Histopathological and Immunohistochemical Characterization of Methly Eugenol-induced Nonneoplastic and Neoplastic Neuroendocrine Cell Lesions in Glandular Stomach of Rats. Toxicologic Pathology. 2015 Jul.



Hormone Receptor Expression in Spontaneous Uterine Adenocarcinoma in Fischer 344 Rats


Willson, C.J., Herbert, R.A., Cline, J.M. (2015) Hormone Receptor Expression in Spontaneous Uterine Adenocarcinoma in Fischer 344 Rats. Toxicologic Pathology. 2015 Jul. 7



JaCVAM-organized international validation study of the in vivo rodent alkaline comet assay


Uno, Y., Kojima, H., Omori, T., Corvi, R., Honma, M., Schechtman, L.M., Tice, R.R., Beevers, C., De Boeck, M., Burlinson, B., Hobbs, C.A., Kitamoto, S., Kraynak, A.R., McNamee, J., Nakagawa, Y., Pant, K., Plappert-Helbig, U., Priestley, C., Takasawa, H., Wada, K., Wirnitzer, U., Asano, N., Escobar, P., Lovell, D., Morita, T., Nakajim, M., Ohno, Y., Hayashi, M. (2015) JaCVAM-organized international validation study of the in vivo rodent alkaline comet assay for detection of genotoxic carcinogens: II. Summary of definitive validation study results. Mutation Research. 2015 Apr. 29



Long noncoding RNA lincRNA-p21 is the major mediator of UVB-induced and p53-dependent apoptosis


Long noncoding RNA lincRNA-p21 is the major mediator of UVB-induced and p53-dependent apoptosis in keratinocytes.Hall JR, Messenger ZJ, Tam HW, Phillips SL, Recio L, Smart RC. Cell Death Dis. 2015 Mar 19



Differential expression of long noncoding RNAs in the livers of female B6C3F1 mice


Differential expression of long noncoding RNAs in the livers of female B6C3F1 mice exposed to the carcinogen furan. Recio L, Phillips SL, Maynor T, Waters M , Jackson AF, Yauk CL. Toxicol Sci. 2013 Oct;135(2):369-79.



Comet assay evaluation of six chemicals of known genotoxic potential in rats


Hobbs CA L. Recio L, Streicker M, Boyle M , Tanaka J, Shiga A, Witt KL. Genet. Toxicol. Environ. Mutagen. (2015).



Methyl Isobutyl Ketone Exposure-Related Increases in Specific Measures of Nephropathy in Male Rats


Borghoff SJ , Poet TS, Green S, Davis J, Hughes B, Mensing T, Sarang SS, Lynch AM, Hard GC. Toxicology. 2015 Mar 19



Gene Expression of Mesothelioma in Vinylidene Chloride-exposed F344/N Rats Reveal Immune Dysfunction


Blackshear PE1, Pandiri AR2, Nagai H3, Bhusari S3, Hong HH3, Ton TV3, Clayton NP3, Wyde M4, Shockley KR5, Peddada SD5, Gerrish KE6, Sills RC3, Hoenerhoff MJ7. Toxicol Pathol. 2014 Jun 23



Predicting chemically-induced skin reactions. Part II: QSAR models of skin permeability


Alves VM, Muratov E, Fourches D, Strickland J, Kleinstreuer NC, Andrade CH, Tropsha A. (2015) Predicting chemically-induced skin reactions. Part II: QSAR models of skin permeability and the relationships between skin permeability and skin sensitization. Toxicology and Applied Pharmacology,



Predicting chemically-induced skin reactions. Part I: QSAR models of skin sensitization


Alves VM, Muratov E, Fourches D, Strickland J, Kleinstreuer NC, Andrade CH, Tropsha A. (2015) Predicting chemically-induced skin reactions. Part I: QSAR models of skin sensitization and their application to identify potentially hazardous compounds. Toxicology and Applied Pharmacology,



t4 workshop report: Integrated Testing Strategies (ITS) for Safety Assessment ALTEX


Rovida C, Alépée N, Api AM, Basketter DA, Bois FY, Caloni F, Corsini E, Daneshian M, Eskes C, Ezendam J, Fuchs H, Hayden P, Hegele-Hartung C, Hoffmann S, Hubesch B, Jacobs MN, Jaworska J, Kleensang A, Kleinstreuer N, Lalko J, Landsiedel R, Lebreux F, Luechtefeld T, Locatelli M, Mehling A, Natsch A, Pitchford JW, Prater D, Prieto P, Schepky A, Schuurmann G, Smirnova L, Toole C, van Vliet E, Weisensee D, Hartung T. 2014. t4 workshop report: Integrated Testing Strategies (ITS) for Safety Assessment ALTEX (in press).



Application of reverse dosimetry to compare in vitro and in vivo estrogen receptor activity


Chang X, Kleinstreuer N, Ceger P, Hsieh J-H, Allen D, Casey W. Application of reverse dosimetry to compare in vitro and in vivo estrogen receptor activity. Applied In Vitro Toxicology (in press).



Immediate and long-term consequences of vascular toxicity during zebrafish development


Tal TL, McCollum CW, Harris PS, Olin J, Kleinstreuer N, Wood CE, Hans C, Shah S, Merchant FA, Bondesson M, Knudsen TB, Padilla S, Hemmer MJ. 2014. Immediate and long-term consequences of vascular toxicity during zebrafish development. Reprod Toxicol 48:51–61.



Open source software implementation of an integrated testing strategy for skin sensitization potency


Pirone JR, Smith M, Kleinstreuer NC, Burns TA, Strickland J, Dancik Y, Morris R, Rinckel LA, Casey W, Jaworska JA. 2014. Open source software implementation of an integrated testing strategy for skin sensitization potency based on a Bayesian network. ALTEX 31: 336–340.



Comments how to make the new vision of toxicity testing in the 21st century a reality


McKim A, Goldberg A, Kleinstreuer N, Busquet F, Andersen M. 2014. Roundtable discussion: Comments on how to make the new vision of toxicity testing in the 21st century a reality. Applied In Vitro Toxicology 1(1): 5–10.



t4 workshop report: Lessons Learned, Challenges, and Opportunities: The U.S. EDSP


Juberg DR, Borghoff SJ, Becker RA, Casey W, Hartung T, Holsapple MP, Marty MS, Mihaich EM, Van Der Kraak G, Wade MG, Willett CE, Andersen ME, Borgert CJ, Coady KK, Dourson ML, Fowle JR, Gray LE, Lamb JC, Ortego LS, Schug TT, Toole CM, Zorrilla LM, Kroner OL, Patterson J, Rinckel LA, Jones BR. 2014. t4 workshop report: Lessons Learned, Challenges, and Opportunities: The U.S. Endocrine Disruptor Screening Program. ALTEX 31:63-78.



Report on the International Workshop on Alternatives to the Murine Histamine Sensitization Test


Isbrucker R, Arciniega J, McFarland R, Chapsal J-M, Xing D, Bache C, Nelson S, Costanzo A, Hoonakker M, Castiaux A, Halder M, Casey W, Johnson N, Jones B, Doelling V, Sprankle C, Rinckel L, Stokes W. 2014. Report on the International Workshop on Alternatives to the Murine Histamine Sensitization Test (HIST) for Acellular Pertussis Vaccines: State of the Science and the Path Forward. Biologicals 42(2):114-122.



Profiling of the Tox21 10K compound library for agonists and antagonists of the estrogen receptor


Huang R, Sakamuru S, Martin MT, Reif DM, Judson RS, Houck KA, Casey W, Hsieh J-H, Shockley KR, Ceger P, Fostel J, Witt KL, Tong W, Rotroff DM, Zhao T, Shinn P, Simeonov A, Dix DJ, Austin CP, Kavlock RJ, Tice RR, Xia M. 2014. Profiling of the Tox21 10K compound library for agonists and antagonists of the estrogen receptor alpha signaling pathway. Nature Scientific Reports



Quantitative profiling of environmental chemicals and drugs that modulate farnesoid X receptor


Hsu CW, Zhao J, Huang R, Hsieh J-H, Hamm J, Chang X, Houck K, M Xia. 2014. Quantitative high-throughput profiling of environmental chemicals and drugs that modulate farnesoid X receptor. Nature Scientific Reports



Case study on the utility of hepatic gene expression profiling in the risk assessment of furan.


Jackson AF, Williams A, Recio L, Waters MD, Lambert IB, Yauk CL. 2014. Case study on the utility of hepatic global gene expression profiling in the risk assessment of the carcinogen furan. Toxicol Appl Pharmacol. 274(1):63-77.



Cytotoxicity of naphthalene toward cells from target and non-target organs in vitro.


Kedderis GL, Shepard KG, Recio. L. 2014. Cytotoxicity of naphthalene toward cells from target and non-target organs in vitro. Chem Biol Interact. 209:85-95.



Relative potency for altered humoral immunity induced by polybrominated & polychlorinated dioxins.


Frawley R, DeVito M, Walker NJ, Birnbaum L, White K Jr, Smith M, Maynor T, Recio L, Germolec D. 2014. Relative potency for altered humoral immunity induced by polybrominated and polychlorinated dioxins/furans in female B6C3F1/N mice. Toxicol Sci. 139(2):488-500.



BrdU treatment to measure hepatocellular proliferation does not mask furan-induced mRNA changes.


Webster AF, Williams A, Recio L, Yauk CL. 2014. Bromodeoxyuridine (BrdU) treatment to measure hepatocellular proliferation does not mask furan-induced gene expression changes in mouse liver. Toxicology. 323C:26-31.



Performance standards and alternative assays: practical insights from skin sensitization.


Kolle SN, Basketter DA, Casati S, Stokes WS, Strickland J, van Ravenzwaay B, Vohr HW, and Landsiedel R. 2013. Performance standards and alternative assays: Practical insights from skin sensitization. Regul Toxicol Pharmacol 65: 278-85.



A computational model predicting disruption of blood vessel development.


Kleinstreuer N, Dix D, Rountree M, Baker N, Sipes N, Reif D, Spencer R, Knudsen T. (2013) A Computational Model Predicting Disruption of Blood Vessel Development. PLoS Comput Biol 9(4): e1002996.



A C. elegans Screening Platform for the Rapid Assessment of Chemical Disruption of Germline Function


Allard P, Kleinstreuer N, Knudsen T, Colaiacovo M. (2013) A novel chemical screening strategy for assessing environmental germline disruption in C. Elegans. Env Health Persp doi:10.1289/ehp.1206301.



Meeting Report: Urinary Pathology; Sixth Research Triangle Park Rodent Pathology Course


Boyle MC, Boyle MH, and the Sixth Rodent Pathology Course Committee. 2013. Meeting Report: Urinary Pathology; Sixth Research Triangle Park Rodent Pathology Course. Vet. Pathol. 50(3): 563-8.



Preparation of archival formalin-fixed paraffin-embedded mouse liver samples for use with the Agilen


Jackson AF, Williams A, Moffat I, Phillips SL, Recio L, Waters MD, Lambert IB, Yauk CL. 2013. Preparation of archival formalin-fixed paraffin-embedded mouse liver samples for use with the Agilent gene expression microarray platform. J Pharmacol Toxicol Methods. S1056-8719(13)00211-6.



Biomarkers of Exposure and Effect in Human Lymphoblastoid TK6 Cells Following [13C2]-Acetaldehyde Ex


Moeller BC, Recio L, Green A, Sun W, Wright FA, Bodnar WM, and Swenberg JA. 2013. Biomarkers of Exposure and Effect in Human Lymphoblastoid TK6 Cells Following [13C2]-Acetaldehyde Exposure. Toxicol Sci. 133(1): 1-12. Editor’s Highlight



Proceedings of the 2012 National Toxicology Program Satellite Symposium.


Elmore S.A., Berridge, B. R., Boyle, M.C., Cora, M.C., Hoenerhoff, M.J., Kooistra, L., Laast, V.A., Morrison, J.P., Rao, D.,Rinke, M. and Yoshizawa, K. 2013. Proceedings of the 2012 National Toxicology Program Satellite Symposium. Toxicol Pathol. 41: 151-180.



Evaluation of the Association between Maternal Smoking, Childhood Obesity, and Metabolic Disorders:


Behl, M., Rao, D., Aagaard, K., Davidson, T.L., Levin, E.D., Slotkin, T.A., Srinivasan, S., Wallinga, D., White, M.F., Walker,V.R., Thayer, K.A., Holloway, A.C. 2013. Evaluation of the Association between Maternal Smoking, Childhood Obesity, and Metabolic Disorders: A National Toxicology Program Workshop Review. Environ Health Perspect. 121(2): 170-180.



Evaluation of 90-day oral rat toxicity studies on the food additive, gum ghatti


Maronpot, R., Davis, J., Moser, G., Giri, D., Hayashi, S. 2012. Evaluation of 90-day oral rat toxicity studies on the food additive, gum ghatti. Food and Chemical Toxicology, 51: 215-224.



Report on the International Workshop on Alternative Methods for Human & Veterinary Rabies Vaccines


Stokes W, McFarland R, Kulpa-Eddy J, Gatewood D, Levis R, Halder M, Pulle G, Kojima H, Casey W, Gaydamaka A, Miller T, Brown K, Lewis C, Chapsal, Bruckner L, Gairola S, Kamphuis E, Rupprecht CE, Wunderli P, McElhinney L, De Mattia F, Gamoh K, Hill R, Reed D, Doelling V, Johnson N, Allen D, Rinckel L, and Jones B. 2012. Report on the International Workshop on Alternative Methods for Human and Veterinary Rabies Vaccine Testing: State of the Science and Planning the Way Forward. Biologicals.



Role of Estrogen Receptor Signaling Required for Endometriosis-like lesion Establishment in mouse


Burns, KA, Rodriguez, KF, Hewitt, SC, Janardhan, KS, Young, SL, Korach, KS. Role of Estrogen Receptor Signaling Required for Endometriosis-like lesion Establishment in a mouse model. Endocrinology Epub June 14 2012.



Proliferative and Non-proliferative Lesions of the Rat and Mouse Central & Peripheral Nervous System


Kaufmann,W., Bolon, B., Bradley, A., Butt, M., Czasch, S., Garman, R.H., George, C., Groters S., Krinke, G., Little, P., McKay, J., Narama, I., Rao, D., Shibutani, M., and Sills, R.A. (2012) Proliferative and Non-proliferative Lesions of the Rat and Mouse Central and Peripheral Nervous Systems. Toxicol Pathol.40: 87S-157S.



Proceedings of the 2011 National Toxicology Program Satellite Symposium


Boorman,G., Crabbs, T.A., Kolenda-Roberts, H., Latimer, K., Miller, a.D., Muravnick, K.B., Nyska, A., Ochoa, r., Pardo, I.d., Ramot, Y., Rao, D.B., Schuh, J., Suttie, A., Travlos, G.S., Ward, J.M., Wolf, J.C., and Elmore, S.A. Proceedings of the 2011 National Toxicology Program Satellite Symposium. Toxicol Pathol. (2012) 40: 321-344.



Bronchial and bronchiolar fibrosis in rats exposed to 2,3-pentanedione vapors


Morgan DL, Jokinen MP, Price HC, Gwinn WM, Palmer SM, Flake GP. Bronchial and bronchiolar fibrosis in rats exposed to 2,3-pentanedione vapors: Implication for bronchiolitis obliterans in humans. Toxicol. Pathol. 40:448-465, 2012.



Morphologic aspects of rodent cardiotoxicity in a restrospective evaluation of National Toxicology P


Jokinen MP, Lieuallen WG, Boyle MC, Johnson CL, Malarkey DE, Nyska A. Morphologic aspects of rodent cardiotoxicity in a restrospective evaluation of National Toxicology Program studies. Toxicol. Pathol. 39:850-860, 2011.



Intestinal Lymphangiectasis and Lipidosis in Rats Following Subchronic Exposure to Indole-3-Carbinol


Michael C. Boyle, Torrie A. Crabbs, Michael E. Wyde, J. Todd Painter, Georgette D. Hill, David E. Malarkey, Warren G. Lieuallen, and Abraham Nyska(2012) Intestinal Lymphangiectasis and Lipidosis in Rats Following Subchronic Exposure to Indole-3-Carbinol via Oral Gavage. Toxicologic Pathology, Vol. 40, No. 4, 561–576 (2012).



Comparison Comet Assay Dose-Response Ethyl Methanesulfonate Freshly Prepared Vs Cryoprepared Tissues


Recio, L., Kissling, G.E., Hobbs, C.A., and Witt, K.L. (2012) Comparison of Comet Assay Dose-Response for Ethyl Methanesulfonate Using Freshly Prepared Versus Cryopreserved Tissues. Environmental and Molecular Mutagenesis 53, 101-113. DOI: 10.1002/em.20694.



Evaluation of the Genotoxicity of the Food Additive, Gum Ghatti


Hobbs, C.A., Swartz, C., Maronpot, R., Davis, J., Recio, L., and Hayashi, S. (2012) Evaluation of the Genotoxicity of the Food Additive, Gum Ghatti. Food and Chemical Toxicology 50, 854-860. DOI: 10.1016/j.fct.2011.11.021.



Genotoxicity of Styrene-Acrylonitrile Trimer in Brain, Liver, and Blood Cells of Weanling F344 Rats


Hobbs, C.A., Chhabra, R.S., Recio, L., Streicker, M. and Witt, K.L. (2012) Genotoxicity of Styrene-Acrylonitrile Trimer in Brain, Liver, and Blood Cells of Weanling F344 Rats. Environmental and Molecular Mutagenesis 53, 227-238. DOI: 10.1002/em.21680



Assessment of Naphthalene-Induced Genotoxicity & Gluathione Detoxication in Human TK6 Lymphoblasts


Recio L, Shepard KG, Hernandez, LG, Kedderis GL. 2012. Dose-Response Assessment of Naphthalene-Induced Genotoxicity and Glutahione Detoxication in Human TK6 Lymphoblasts. Toxicol. Sci. 126(2):405-412.



Therapeutic Ultrasound as a Potential Male Contraceptive


Tsuruta JK, Dayton PA, Gallippi CM, O’Rand MG, Streicker MA, Gessner, RC, Gregory TS, Silva E JR, Hamil, KG, Moser GJ, Sokai, DC. 2012. Therapeutic Ultrasound as a Potential Male Contraceptive: Power, Frequency and Temperature Required to Deplete Rat Testes of Meiotic Cells and Epididymides of Sperm Determined Using Commercially Available System. Reproductive Biology and Endocrinology 10:7.



15-Day Intact Male Rat Assay: Evaluation of an Antithyroid Chemical and a Negative Control Chemical


Becker RA, Bergfelt, DR, Borghoff S, Davis JP, Hamby BT, O’Connor JC, Kaplan AM, Sloan CS, Tyl, RW, Wade M, Marty MS. 2012. Interlaboratory Study Comparison of the 15-Day Intact Adult Male Rat Screening Assay: Evaluation of an Antithyroid Chemical and a Negative Control Chemical. Birth Defects Research (Part B). (95):63-78.



Improving Animal Welfare and Reducing Animal Use for Veterinary Vaccine Potency Testing


Stokes WS, Brown K, Kulpa-Eddy J, Srinivas G, Halder M, Draayer H, Galvin J, Claasen I, Gifford G, Woodland R, Doelling V, Jones B. 2011. Improving Animal Welfare and Reducing Animal Use for Veterinary Vaccine Potency Testing: State of the Science and Future Directions. Procedia in Vaccinology(5):84-105.



Alt. Methods to Reduce, Refine & Replace Animal Use for Veterinary Vaccine Post-Licensing Testing


Kulpa-Eddy J, Srinivas G, Halder M, Brown K, Draayer H, Glavin J, Claassen I, Gifford G, Woodland R, Doelling V, Jones, B, Stokes WS. 2011. Alternative Methods and Strategices to Reduce, Refine and Replace Animal Use for Veterinary Vaccine Post-Licensing Safety Testing: State of the The Science and Future Directions.Procedia in Vaccinology(5):106-119.



Alternative Methods to Reduce, Refine & Replace Animal Use for Human Vaccine Post-Licensing Testing


Isbrucker R, Levis R, Casey W, McFarland R, Schmitt M, Arciniega J, Descamps J, Finn T, Hendriksen C, Horiuchi Y, Keller J, Kojima H, Sesardic D, Stickings P, Johnson NW, Allen D. 2011. Alternative Methods and Strategies to Reduce, Refine and Replace Animal Use for Human Vaccine Post-Licensing Safety Testing: State of the Science and Future Directions. Procedia in Vaccinology(5):47-59.



Non-Animal Replacement Methods for Veterinary Vaccine Potency Testing


Kulp-Eddy J, Srinivas G, Halder M, Hill R, Brown K, Roth J, Draayer H, Galvin G, Woodland R, Doelling V, Jones B, Stokes WS. 2011. Non-Animal Replacement Methods for Veterinary Vaccine Potency Testing: State of the Science and Future Directions. Procedia in Vaccinology(5):60-83.



Improving Animal Welfare and Reducing Animal Use for Human Vaccine Potency Testing


Casey W, Schmitt M, McFarland R, Isbrucker R, Levis R, Arciniega J, Descamps J, Finn T, Hendriksen C, Horluchi Y, Keller, J, Kojima H, Sesardic D, Stickings P, Johnson NW, Lipscomb E, Allen D. 2011. Improving Animal Welfare Reducing Animal Use for Human Vaccine Potency Testing: State of the Science and Future Directions. Procedia in Vaccinology(5):33-46.



Non-Animal Replacement Methods for Human Potency Testing: State of the Science and Future Directions


McFarland R, Verthelyi D, Casey W, Arciniega J, Isbrucker R, Schmitt M, Finn T, Descamps J, Horiuchi Y, Sesardic D, Stickings P, Johnson NW, Lipscomb E, Allen D. 2011. Non-Animal Replacement Methods for Human Vaccine Potency Testing: State of the Science and Future Directions. Procedia in Vaccinology(5): 16-32.



Evaluation of the Genotoxicity of the Food Additive, Gum Ghatti


Hobbs CA, Swartz C, Maronpot R, Davis J, Recio L, and Hayashi S. 2011. Evaluation of the Genotoxicity of the Food Additive, Gum Ghatti. Food and Chemical Toxicology DOI:10.1016/j.fct.2011.11.021.



Improvement of In Vivo Genotoxicity: Acute Tests and Integration into Standard Toxicity Testing


Rothfuss A, Honma M, Czich A, Aardema MJ, Burlinson B, Galloway S, Hamada S, Kirkland D, Heflich RH, Howe J, Nakajima M, O’Donovan M, Plappert-Helbig U, Priestley C, Recio L, Schuler M, Uno Y, and Martus HJ. 2011. Improvement of In Vivo Genotoxicity: Combination of Acute Tests and Integration into Standard Toxicity Testing. Mutat. Res. 723: 108-120.



Comet Assay Dose-Response for Ethyl Methanesulfonate Using Freshly Prepared Vs. Cryopreserved Tissue


Recio L, Kissling GE, Hobbs CA, and Witt KL, 2011. Comparison of Comet Assay Dose-Response for Ethyl Methansulfonate Using Freshly Prepared Versus Cryopreserved Tissues. Environmental and Molecular Mutagenesis (in press).



Using Fewer Animals to Identify Chemical Eye Hazards: Criteria to Maintain Hazard Classification


Haseman JK,Allen DG,Lipscomb EA,Truax JF,Stokes WS. 2011. Using Fewer Animals to Identify Chemical Eye Hazards: Revised Criteria Necessary to Maintain Equivalent Hazard Classification. Regulatory Toxicology and Pharmacology 61:98-104.



Disseminiated Histoplasmosis with Concurrent Oral Candidiasis in an Eclectus Parrot


Quist EM,Belcher C,Levine G,Johnson M,Heatley JJ,Kiupel M, Giri D. 2011. Disseminated Histoplasmosis with Concurrent Oral Candidiasis in an Electus Parrot (Eclectus Roratus). Avian Pathol. 40(3):339.



Cerebral and Renal Phaeohyphomycosis in a Dog Infected with Bipolaris Species


Giri DK,Sims WP,Sura R,Cooper JJ,Gavrilov BK,Mansell J. 2011. Cerebral and Renal Phaeohhyphomycosis in a Dog Infected with Bipolaris Species. Vet Pathol. 48(3):754-7.



Neoplastic and Nonneoplastic Liver Lesions Induced by Dimethylnitrosamine in Japanese Medaka Fish


Hobbie KR,DeAngelo AB,George MH,Law JM. 2011. Neoplastic and Nonneoplastic Liver Lesion Induced by Dimethylnitrosamine in Japanes Medaka Fish. Vet. Pathol. Online. DOI: 10.1177/0300985811409443.



Liver Toxicity and Carcinogenicity in f344/n Rats and b6cf1 Mice Exposed to Kava Kava


Behl M,Nyska A,Chhabra RS,Travlos G,Fomby LM,Sparrow BR,Hejtmancik MR,Chan PC. 2011. Liver Toxicity and Carcinogenicity in f344/n Rats and b6c3f1 Mice Exposed to Kava Kava. Food and Chemical Toxicology. In press for publication in November 2011.



Safety Assessment of Allergic Contact Dermatitis Hazards:  An Analysis Supporting Reduced Animal Use


Haseman JK,Strickland J,Allen D,Salicru E,Paris M,Tice RR,Stokes WS. 2011. Safety Assessment of Allergic Contact Dermatitis Hazards: An Analysis Supporting Reduced Animal Use for the Murine Local Lymph Node Assay. Regulatory Toxicology and Pharmacology 59: 191-96.



Assessment of a Regulated AAV2 Gene Transfer Vector After Murine Submandibular Glands Delivery


Zheng C,Voutetakis A,Goldstein B,Afione S,Rivera V,Clackson T,Wenk M,Boyle M,Nyska A,Chiorini J,Vallant M,Irwin R,Baum B. 2011. Assessment of the Safety and Biodistribution of a Regulated AAV2 Gene Transfer Vector After Delivery to Murine Submandibular Glands. Toxicological Sciences.



Effects of Atrazine on Steroidgenesis in Rat Granulosa and H295R Adrenal Cortical Carcinoma Cells


Tinfo NS,Hotchkiss MG,Buckalew AR,Zorrilla LM,Cooper RL,Laws SC. 2011. Understanding the Effects of Atrazine on Steriodogenesis in Rat Granulosa and H295 Adrenal Cortical Carcinoma Cells.Reprod. Toxicol. 31(2):184-93



Histopathological Evaluation of the Nervous System in National Toxicology Program of Rodent Studies


Rao, D, Little, PB, Malarkey, D.E, Herbert, RA, Sills, RC. 2011. Histopathological Evaluation of the Nervous System in National Toxicology Program of Rodent Studies: A Modified Approach. Toxicologic Pathology 39: 463-470.



Genetic Toxicology in the 21st Century: Reflections and Future Directions


Mahadevan B, Snyder R, Waters M, Benz RD, Kemper RA, Tice RR and Richard AM. 2011. Genetic Toxicology in the 21st Century: Reflections and Future Directions. Environ. Mol. Mutagen. 52.



Effects of Hormonally Active Agents on Steroid Hormone Receptor Expression and Cell Proliferation


Hill GD, Moore AB, Kissling GE, Flagler ND, Ney E, Cline JM, Dixon D. 2011. Effects of Hormonally Active Agents on Steroid Hormone Receptor Expression and Cell Proliferation in the Myometrium of Ovariectomized Macaques. Toxicol. Pathol. 39(3): 508-515.



Toxicity Study of Green Tea Extract in Rats and Mice


Chan PC, Ramot Y, Malarkey DE, Blackshear P, Kissling GE, Travlos G, Nyska A. 2010. Fourteen week Toxicity Study of Green Tea Extract in Rats and Mice. Toxicol. Pathol. 38(7): 1070-1084.



Characterizing and Predicting Carcinogenicity and Mode of Action


Waters M, Jackson MA, and Lea IA. 2010. Characterizing and predicting carcinogenicity and mode of action using conventional and toxicogenomics methods. Mutat Res 705: 184-200.



Dose-Response of Four Genotoxic Chemicals in a Combined Mouse and Rat Micronucleus and Comet Assay


Recio L, Hobbs CA, Caspary W, Witt KL. 2010. Dose-Response Assessment of Four Genotoxic Chemicals in a Combined Mouse and Rat Micronucleus and Comet Assay Protocol. Toxicol Sciences 35(2): 149-162.



No Biomarkers of Genetic Damage or Pathological Changes in Male Rats Administered Ritalin


Witt KL, Malarkey DE, Hobbs CA, Davis JP, Kissling GE, Caspary W, Travlos G, Recio L. 2010. No increases in biomarkers of genetic damage or pathological changes in heart and brain tissues in male rats administered methylphenidate hydrochloride (Ritalin) for 28 days. Environ Mol Mutagen 51(1): 80-88.